Dkk-4 Antibody (185709) [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: WB, ELISA, ELISA(Cap), ELISA(Det)
• Clone: 185709
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 669

Dkk-4 Antibody (185709) [Janelia Fluor® 669] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Dkk-4
  Leu19-Leu224
  Accession # Q9UBT3
• Specificity: Detects human Dkk-4 in ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant mouse (rm) Dkk-4 is observed and no cross-reactivity with recombinant human (rh) Dkk-1 or rhDkk-3 is observed. In sandwich immunoassays, less than 2% cross-reactivity with rmDkk-4 is observed and no cross-reactivity with rhDkk-1, recombinant rat Dkk-1, rmDkk‑1, or rhDkk-3 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DKK4
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Dkk-4 Antibody (185709) [Janelia Fluor® 669]

• dickkopf (Xenopus laevis) homolog 4
• dickkopf homolog 4 (Xenopus laevis)
• dickkopf-4
• dickkopf-related protein 4
• Dkk4
• Dkk-4
• hDkk-4
• MGC129562
• MGC129563

Background

DKK4 is a secreted protein that is a member of the dickkopf family. Like some other members of the dickkopf family DKK4 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The activity of DKK4 is modulated by binding to the Wnt co-receptor and the co-factor kremen 2, and it is proposed that it is also an inhibitor of the Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DKK4