Dkk-3 Overexpression Lysate (Native)

Images

 
Western Blot: DKK3 Overexpression Lysate (Adult Normal) [NBL1-09904] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DKK3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Dkk-3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200199 and based on accession number NM_013253. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dickkopf homolog 3 (Xenopus laevis) (DKK3), transcript variant 2, mRNA.
Gene
DKK3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
36.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Dkk-3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Dkk-3 Overexpression Lysate (Native)

  • dickkopf (Xenopus laevis) homolog 3
  • dickkopf 3
  • dickkopf homolog 3 (Xenopus laevis)
  • Dickkopf-3
  • dickkopf-related protein 3
  • Dkk3
  • Dkk-3
  • hDkk-3
  • regulated in glioma
  • REIC
  • REICdkk-3
  • RIG
  • RIG-like 5-6
  • RIG-like 7-1

Background

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1384
Species: Hu
Applications: WB, IHC, ICC
1341-WF/CF
Species: Hu
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
AF2435
Species: Mu
Applications: WB, IHC
1169-FR/CF
Species: Mu
NBP2-27109
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western, IHC, IHC-P
NBP2-20331
Species: Hu
Applications: WB, IHC, IHC-P
1827-SF/CF
Species: Hu
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
1269-DK/CF
Species: Hu
NBP2-33717
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-1244
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
MAB6798
Species: Hu
Applications: WB, ICC
NBP1-86856
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-83224
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-09904
Species: Hu
Applications: WB

Publications for Dkk-3 Lysate (NBL1-09904) (0)

There are no publications for Dkk-3 Lysate (NBL1-09904).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Dkk-3 Lysate (NBL1-09904) (0)

There are no reviews for Dkk-3 Lysate (NBL1-09904). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Dkk-3 Lysate (NBL1-09904) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Dkk-3 Products

Bioinformatics Tool for Dkk-3 Lysate (NBL1-09904)

Discover related pathways, diseases and genes to Dkk-3 Lysate (NBL1-09904). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Dkk-3 Lysate (NBL1-09904)

Discover more about diseases related to Dkk-3 Lysate (NBL1-09904).
 

Pathways for Dkk-3 Lysate (NBL1-09904)

View related products by pathway.

PTMs for Dkk-3 Lysate (NBL1-09904)

Learn more about PTMs related to Dkk-3 Lysate (NBL1-09904).
 

Research Areas for Dkk-3 Lysate (NBL1-09904)

Find related products by research area.

Blogs on Dkk-3

There are no specific blogs for Dkk-3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Dkk-1 [Unconjugated]
5439-DK-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Dkk-3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DKK3
Entrez