Dkk-3 Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

Dkk-3 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Dkk-3
  Met1-Ile349
  Accession # Q9QUN9
• Specificity: Detects mouse Dkk-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Dkk‑3 is observed and less than 1% cross-reactivity with rhDkk-1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-3 Antibody [Alexa Fluor® 750]

• dickkopf (Xenopus laevis) homolog 3
• dickkopf 3
• dickkopf homolog 3 (Xenopus laevis)
• Dickkopf-3
• dickkopf-related protein 3
• Dkk3
• Dkk-3
• hDkk-3
• regulated in glioma
• REIC
• REICdkk-3
• RIG
• RIG-like 5-6
• RIG-like 7-1

Background

Dkk-3 (Dickkopf-related protein 3; also REIC) is a 65-70 kDa secreted glycoprotein member of the Dickkopf family of molecules.  It is expressed by multiple cell types, including neurons, endothelial cells, keratinocytes and zona glomerulosa cells of the adrenal.  Dkk-3 is reported to be a positive regulator of Wnt signaling.  It is suggested to bind exclusively to Wnt-inhibiting Kremen proteins, thereby blocking their expression on the cell surface.  This makes LRP available for Wnt binding and signaling.  Dkk-3 is also reported to induce the development of DC from monocytes.  Mature mouse Dkk-3 is 328 amino acids (aa) in length (aa 22-349).  It contains one coiled-coil region (aa 39-84), two consecutive Dkk-type Cys-rich regions (aa 147-195 and 208-284), and one phosphoserine site at Ser298.  Over aa 22-349, mouse shares 83% and 92% aa identity with human and rat Dkk-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.