DKC1 Antibody

Images

 
Immunohistochemistry-Paraffin: DKC1 Antibody [NB100-59738] - IHC analysis of DKC1 in paraffin embedded Human Spleen using this antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DKC1 Antibody Summary

Immunogen
Peptide with sequence C-KRKRESESESDETPP corresponding to internal region according to NP_001354.1.
Epitope
KRKRESESESDETPP
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 70-75 kDa band in human bone marrow, duodenum and skin lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7 kDa band according to NP_001354.1. The 70-75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human spleen shows strong nuclear staining of select splenocytes.
Positive Control
DKC1 Lysate (NBL1-09897)
Publications
Read Publication using NB100-59738.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DKC1 Antibody

  • CBF5 homolog
  • CBF5
  • cbf5p homolog
  • DKC
  • dyskeratosis congenita 1, dyskerin
  • Dyskerin
  • EC 5.4.99
  • EC 5.4.99.-
  • FLJ97620
  • H/ACA ribonucleoprotein complex subunit 4
  • NAP57
  • NOLA4dyskerin
  • Nopp140-associated protein of 57 kDa
  • Nucleolar protein family A member 4
  • Nucleolar protein NAP57
  • snoRNP protein DKC1
  • XAP101

Background

DKC1 (Dyskeratosis congenita 1) is a nucleolar protein, altered in dyskeratosis congenita, which carries out two separate functions, both fundamental for proliferating cells. One function is the pseudouridylation of ribosomal RNA (rRNA) molecules, necessary for their processing, and the other is the stabilization of the telomerase RNA component, necessary for telomerase activity. It is thought to be the catalytic subunit of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. DKC1 is also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Both 18S rRNA production and rRNA pseudouridylation are impaired if DKC1 is depleted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-317
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-32441
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13656
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-31742
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84423
Species: Hu
Applications: IHC, IHC-P
NBP2-57100
Species: Hu
Applications: ICC/IF
NB600-1522
Species: Hu, Mu
Applications: WB, GS, ICC/IF
NBP1-58200
Species: Hu, Ze
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NBP1-89306
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-92388
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-68252
Species: Hu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KO
H00004354-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP1-81681
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77306
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
NBP1-83837
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-59738
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA

Publications for DKC1 Antibody (NB100-59738)(1)

Reviews for DKC1 Antibody (NB100-59738) (0)

There are no reviews for DKC1 Antibody (NB100-59738). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DKC1 Antibody (NB100-59738) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional DKC1 Products

Bioinformatics Tool for DKC1 Antibody (NB100-59738)

Discover related pathways, diseases and genes to DKC1 Antibody (NB100-59738). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DKC1 Antibody (NB100-59738)

Discover more about diseases related to DKC1 Antibody (NB100-59738).
 

Pathways for DKC1 Antibody (NB100-59738)

View related products by pathway.

PTMs for DKC1 Antibody (NB100-59738)

Learn more about PTMs related to DKC1 Antibody (NB100-59738).

Blogs on DKC1

There are no specific blogs for DKC1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DKC1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DKC1
Entrez
Uniprot