Dihydrofolate Reductase/DHFR Antibody

Images

 
Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGE DHFR antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: Dihydrofolate reductase Antibody [NBP2-16163] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Dihydrofolate reductase Antibody [NBP2-16163] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Western Blot: Dihydrofolate reductase Antibody [NBP2-16163] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:1000.
Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 ug of DHFR antibody. Western blot analysis was performed using DHFR ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

Dihydrofolate Reductase/DHFR Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DHFR. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHFR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (88%), Bovine (85%), Porcine (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dihydrofolate Reductase/DHFR Antibody

  • DHFR
  • DHFRP1
  • Dihydrofolate Reductase
  • DYR
  • EC 1.5.1.3

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DHFR
Entrez
Uniprot