![Western Blot: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Western blot analysis of Dihydrofolate Reductase/DHFR on different lysates with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/1,000 dilution. Lane 1: HCT116 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: U-2 OS cell lysate (15 ug/Lane) Lane 4: K-562 cell lysate (15 ug/Lane) Lane 5: MCF7 cell lysate (15 ug/Lane) Lane 6: HEK-293 cell lysate (15 ug/Lane) Lane 7: Jurkat cell lysate (15 ug/Lane) Lane 8: HepG2 cell lysate (15 ug/Lane) Lane 9: LO2 cell lysate (15 ug/Lane) Lane 10: Mouse liver tissue lysate (30 ug/Lane) Lane 11: Rat liver tissue lysate (30 ug/Lane) Predicted band size: 21.5 kDa Observed band size: 21.5 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32268) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32268_rabbit-dihydrofolate-reductase-dhfr-mab-je31-05-278202416331265.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Western blot analysis of Dihydrofolate Reductase/DHFR on different lysates with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/1,000 dilution. Lane 1: HCT116 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: U-2 OS cell lysate (15 ug/Lane) Lane 4: K-562 cell lysate (15 ug/Lane) Lane 5: MCF7 cell lysate (15 ug/Lane) Lane 6: HEK-293 cell lysate (15 ug/Lane) Lane 7: Jurkat cell lysate (15 ug/Lane) Lane 8: HepG2 cell lysate (15 ug/Lane) Lane 9: LO2 cell lysate (15 ug/Lane) Lane 10: Mouse liver tissue lysate (30 ug/Lane) Lane 11: Rat liver tissue lysate (30 ug/Lane) Predicted band size: 21.5 kDa Observed band size: 21.5 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32268) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | JE31-05 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Dihydrofolate Reductase/DHFR Antibody (JE31-05) (NBP3-32268) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human Dihydrofolate Reductase/DHFR aa 88-187 / 187. (Uniprot: P00374) |
| Localization | Mitochondrion, Cytoplasm. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | DHFR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 21.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Dihydrofolate Reductase/DHFR Antibody (NBP3-32268)Find related products by research area.
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| Gene Symbol | DHFR |
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32268) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32268_rabbit-dihydrofolate-reductase-dhfr-mab-je31-05-298202413585558.jpg "Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32268) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry TFF2 Antibody (366508) [Unconjugated]](https://images.novusbio.com/images2/TFF2_MAB4077_Immunohistochemistry_21485.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")