DIAPH1 Antibody

Western Blot: DIAPH1 Antibody [NBP1-47202] - Analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47202 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DIAPH1 Antibody Summary

• Description: Novus Biologicals Goat DIAPH1 Antibody (NBP1-47202) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QTKTSKAKKDQ corresponding to internal region according to NP_005210.3, NP_001073280.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DIAPH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 140 kDa band observed in NIH3T3 lysates (calculated MW of 141 kDa band according to human NP_005210.3 and 139 kDa band according to mouse NP_031884.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DIAPH1 Antibody

• DIA1
• diaphanous homolog 1 (Drosophila)
• hDIA1
• homolog) 1
• LFHL1FLJ25265

Background

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIAPH1
• Entrez:
  • 1729(Human)
  • 13367(Mouse)
• Uniprot:
  • NP_005210.3(Human)