DIAPH1 Antibody

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Western Blot: DIAPH1 Antibody [NBP1-47202] - Analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47202 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DIAPH1 Antibody Summary

Immunogen
Peptide with sequence C-QTKTSKAKKDQ corresponding to internal region according to NP_005210.3, NP_001073280.1.
Epitope
internal region
Specificity
This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DIAPH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 140 kDa band observed in NIH3T3 lysates (calculated MW of 141 kDa band according to human NP_005210.3 and 139 kDa band according to mouse NP_031884.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DIAPH1 Antibody

  • DIA1
  • diaphanous homolog 1 (Drosophila)
  • hDIA1
  • homolog) 1
  • LFHL1FLJ25265

Background

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DIAPH1
Entrez
Uniprot