DHX8 Antibody

Images

 
Western Blot: DHX8 Antibody [NB100-1714] - Detection of Human DHX8 on HeLa whole cell lystate using NB100-1714. DHX8 was also immunoprecipitated using rabbit anti-DHX8 antibodies NB100-1713 and NB100-1715.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

DHX8 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 using the numbering given in entry NP_004932.1 (GeneID 1659).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHX8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 1-4 ug/mg of lysate
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DHX8 Antibody

  • DDX8ATP-dependent RNA helicase DHX8
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
  • DEAH (Asp-Glu-Ala-His) box polypeptide 8
  • DEAH box protein 8
  • DEAH-box protein 8
  • EC 3.6.1
  • EC 3.6.4.13
  • HRH1RNA helicase HRH1
  • PRP22
  • PRPF22

Background

DHX8 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX8 is the human ortholog of the yeast Prp22 protein. DHX8 has been demonstrated to function in the release of RNA from the splicesome and the nuclear export of spliced mRNA. Alternate names for DHX8 include ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1, DDX8, HRH1, PRP22, and PRPF22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DHX8
Entrez
Uniprot