DHX8 Antibody (1E10)

Western Blot: DHX8 Antibody (1E10) [H00001659-M01] - DHX8 monoclonal antibody (M01), clone 1E10 Analysis of DHX8 expression in Hela S3 NE.

Sandwich ELISA: DHX8 Antibody (1E10) [H00001659-M01] - Detection limit for recombinant GST tagged DHX8 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, S-ELISA
• Clone: 1E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DHX8 Antibody (1E10) Summary

• Immunogen: DHX8 (NP_004932, 301 a.a. ~ 400 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKW
• Specificity: DHX8 (1E10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DHX8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DHX8 Antibody (1E10)

• DDX8ATP-dependent RNA helicase DHX8
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
• DEAH (Asp-Glu-Ala-His) box polypeptide 8
• DEAH box protein 8
• DEAH-box protein 8
• EC 3.6.1
• EC 3.6.4.13
• HRH1RNA helicase HRH1
• PRP22
• PRPF22

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHX8
• Entrez:
  • 1659(Human)
• OMIM:
  • 600396(Human)
• Uniprot:
  • NP_004932(Human)