Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human CHRM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

CHRM3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 580011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CHRM3
  Accession # P20309
• Specificity: Detects HEK293 cells transfected with human CHRM3 in Cell-based ELISA
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHRM3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated]

• cholinergic receptor, muscarinic 3
• CHRM3
• HM3
• m3 muscarinic receptor
• mAChR M3
• muscarinic acetylcholine receptor M3

Background

Muscarinic acetylcholine receptor M3 (CHRM3) is a 65 kDa 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Human CHRM3 shares 92% aa sequence identity with mouse and rat CHRM3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHRM3
• Uniprot:
  • P20309()