Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated]

Images

 
HEK293 human embryonic kidney cell line transfected with (A) human CHRM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
580011
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human CHRM3
Accession # P20309
Specificity
Detects HEK293 cells transfected with human CHRM3 in Cell-based ELISA
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CHRM3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated]

  • cholinergic receptor, muscarinic 3
  • CHRM3
  • HM3
  • m3 muscarinic receptor
  • mAChR M3
  • muscarinic acetylcholine receptor M3

Background

Muscarinic acetylcholine receptor M3 (CHRM3) is a 65 kDa 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Human CHRM3 shares 92% aa sequence identity with mouse and rat CHRM3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHRM3
Uniprot