DHODH Antibody (6E1) - Azide and BSA Free

Western Blot: DHODH Antibody (6E1) [H00001723-M01] - DHODH monoclonal antibody (M01), clone 6E1 Analysis of DHODH expression in MCF-7.

Immunohistochemistry-Paraffin: DHODH Antibody (6E1) [H00001723-M01] - Analysis of monoclonal antibody to DHODH on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.

Sandwich ELISA: DHODH Antibody (6E1) [H00001723-M01] - Detection limit for recombinant GST tagged DHODH is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 6E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DHODH Antibody (6E1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DHODH (NP_001352, 32 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQ
• Specificity: DHODH - dihydroorotate dehydrogenase
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: DHODH
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DHODH Antibody (6E1) - Azide and BSA Free

• DHOdehase
• DHODH
• dihydroorotate dehydrogenase
• dihydroorotate dehydrogenase, mitochondrial
• Dihydroorotate Oxidase
• EC 1.3.3.1
• EC 1.3.5.2
• human complement of yeast URA1
• POADS
• URA1

Background

The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DHODH Antibody (H00001723-M01)(1)

Publication using H00001723-M01

• Lucas-Hourani M, Dauzonne D, Jorda P et al. Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity. PLoS Pathog. 2013-10-03 [PMID: 24098125]

Bioinformatics

• Gene Symbol: DHODH
• Entrez:
  • 1723(Human)
• OMIM:
  • 126064(Human)
• Uniprot:
  • NP_001352(Human)