DGK-epsilon Antibody (670914) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 670914
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

DGK-epsilon Antibody (670914) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human DGK-epsilon
  Asn314-Arg435
  Accession # P52429
• Specificity: Detects human DGK-epsilon in direct ELISAs and human, mouse and rat DGK-epsilon in Western blots. In Western blots, approximately 10-50% cross-reactivity with recombinant human DGK zeta, eta, iota, alpha, theta, gamma, kappa, delta, and beta is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-epsilon Antibody (670914) [Alexa Fluor® 750]

• DAG kinase epsilon
• DAGK5
• DAGK6
• DGK
• DGKE
• DGKepsilon
• DGK-epsilon
• diacylglycerol kinase epsilon
• diacylglycerol kinase, epsilon (64kD)
• diacylglycerol kinase, epsilon 64kDa
• Diglyceride kinase epsilon
• EC 2.7.1.107

Background

DGK-epsilon (Diacylglycerol kinase epsilon) is a 65 kDa member of the eukaryocytic diacylglycerol kinase family of enzymes. It is a type III DGK that possesses only a C1/Cys-rich domain and a catalytic region, and is found in neurons and testis. DGK-epsilon specifically phosphorylates arachidonate-containing DAG, and may downregulate DAG signaling that results from inositol cycling. Human DGK-epsilon is 567 amino acids (aa) in length. It contains one predicted transmembrane domain (aa 22-42), two C1 DAG-binding regions (aa 59-108 and 124-177) and one catalytic domain (aa 219-350). DGK-epsilon is predicted to form intramembrane oligomers. Over aa 314-435, human DGK-epsilon shares 99% aa identity with mouse DGK-epsilon .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P52429()