DGK-epsilon Antibody (670914) [Alexa Fluor® 700]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 670914
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

DGK-epsilon Antibody (670914) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human DGK-ε
  Asn314-Arg435
  Accession # P52429
• Specificity: Detects human DGK-epsilon in direct ELISAs and human, mouse and rat DGK-epsilon in Western blots. In Western blots, approximately 10-50% cross-reactivity with recombinant human DGK zeta, eta, iota, alpha, theta, gamma, kappa, delta, and beta is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-epsilon Antibody (670914) [Alexa Fluor® 700]

• DAG kinase epsilon
• DAGK5
• DAGK6
• DGK
• DGKE
• DGKepsilon
• DGK-epsilon
• diacylglycerol kinase epsilon
• diacylglycerol kinase, epsilon (64kD)
• diacylglycerol kinase, epsilon 64kDa
• Diglyceride kinase epsilon
• EC 2.7.1.107

Background

DGK-epsilon (Diacylglycerol kinase epsilon) is a 65 kDa member of the eukaryocytic diacylglycerol kinase family of enzymes. It is a type III DGK that possesses only a C1/Cys-rich domain and a catalytic region, and is found in neurons and testis. DGK-epsilon specifically phosphorylates arachidonate-containing DAG, and may downregulate DAG signaling that results from inositol cycling. Human DGK-epsilon is 567 amino acids (aa) in length. It contains one predicted transmembrane domain (aa 22-42), two C1 DAG-binding regions (aa 59-108 and 124-177) and one catalytic domain (aa 219-350). DGK-epsilon is predicted to form intramembrane oligomers. Over aa 314-435, human DGK-epsilon shares 99% aa identity with mouse DGK-epsilon .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.