DGCR8 knockout MEF cells

Immunocytochemistry/Immunofluorescence: DGCR8 knockout MEF cells [NBP2-25171] - The DGCR8 knockout MEF cells were stained for alpha Tubulin at a 1:1000 dilution against Dylight 550 (Red) to show composition of cell structure. Nuclei were counterstained with DAPI (blue)

In vitro assay: DGCR8 knockout MEF cells [NBP2-25171] - Brightfield Image of DGCR8 MEF knockout cells

• Reactivity: Mu
• Applications: Func

DGCR8 knockout MEF cells Summary

• Gene: DGCR8

Applications/Dilutions

• Dilutions:
  • In vitro assay
• Application Notes: Please see Protocol for culturing conditions.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28102192)

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Buffer: Cells are supplied in 1.5 ml quantities (about 1x10 ^ 6 cells/ml) in freezing media (80% MEF media, 10% FBS, 10% DMSO)

Alternate Names for DGCR8 knockout MEF cells

• C22orf12
• DGCRK6
• Gy1
• pasha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for DGCR8 knockout MEF cells (NBP2-25171)(5)

Publications using NBP2-25171

• M Cesana, MH Guo, D Cacchiarel, L Wahlster, J Barragan, S Doulatov, LT Vo, B Salvatori, C Trapnell, K Clement, P Cahan, KM Tsanov, PM Sousa, B Tazon-Vega, A Bolondi, FM Giorgi, A Califano, JL Rinn, A Meissner, JN Hirschhorn, GQ Daley A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development Cell Stem Cell, 2018-04-05;22(4):575-588.e7. 2018-04-05 [PMID: 29625070]
• Calses PC, Dhillon KK, Tucker N et al. DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing. Cell Rep. 2017-04-04 [PMID: 28380355] (In vitro)
• Gambardella G, Carissimo A, Chen A et al. The impact of microRNAs on transcriptional heterogeneity and gene co-expression across single embryonic stem cells Nat Commun 2017-01-19 [PMID: 28102192] (FLOW, Mouse)
• Rasschaert P, Figueroa T, Dambrine G et al. Alternative splicing of a viral mirtron differentially affects the expression of other microRNAs from its cluster and of the host transcript. RNA Biol 2016-10-07 [PMID: 27715458] (WB)
• Krawczynski K, Najmula J, Bauersachs S, Kaczmarek MM. MicroRNAome of porcine conceptuses and trophoblasts: expression profile of micrornas and their potential to regulate genes crucial for establishment of pregnancy. Biol Reprod. [PMID: 25472924]

Bioinformatics

• Gene Symbol: DGCR8