DFF40/CAD Antibody - BSA Free

Western Blot: DFF40/CAD Antibody [NBP1-76884] - Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution.

Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-76884] - Jurkat cells with DFF40 antibody at 5 ug/mL.

Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-76884] - Immunofluorescence of DFF40 in Jurkat cells with DFF40 antibody at 20 ug/mL.

Product Discontinued

DFF40/CAD Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid peptide near the amino terminus of human DFF40. The immunogen is located within the first 50 amino acids of DFF40.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DFFB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: DFF40 antibody can be used for detection of DFF40 by Western blot at 1:500 to 1:2000 dilution. A 40 kDa band can be detected.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DFF40/CAD Antibody - BSA Free

• CAD
• CADCaspase-activated nuclease
• Caspase-activated deoxyribonuclease
• Caspase-activated DNase
• CPAN
• CPANDNA fragmentation factor 40 kDa subunit
• DFF2
• DFF40
• DFF-40DFF40DFF2DNA fragmentation factor subunit beta
• DFFB
• DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• EC 3.-

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for DFF40/CAD Antibody (NBP1-76884). (Showing 1 - 1 of 1 FAQ).

1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
   • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Bioinformatics

• Gene Symbol: DFFB
• Uniprot:
  • NP_004393(Human)