Desmoglein-3 Antibody (1T1D5)

Western Blot: Desmoglein-3 Antibody (1T1D5) [NBP3-16597] - Western blot analysis of extracts of various cell lines, using Desmoglein-3 Rabbit mAb (NBP3-16597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (1T1D5) [NBP3-16597] - Immunofluorescence analysis of NIH-3T3 cells using Desmoglein-3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 1T1D5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Desmoglein-3 Antibody (1T1D5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 900-999 of human Desmoglein-3 (P32926). TLSGSQGASALSTSGSVQPAVSIPDPLQHGNYLVTETYSASGSLVQPSTAGFDPLLTQNVIVTERVICPISSVPGNLAGPTQLRGSHTMLCTEDPCSRLI
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DSG3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Desmoglein-3 Antibody (1T1D5)

• Cadherin family member 6
• CDHF6
• CDHF6130-kD pemphigus vulgaris antigen
• desmoglein 3 (pemphigus vulgaris antigen)
• desmoglein 3
• Desmoglein3
• Desmoglein-3
• DKFZp686P23184,130 kDa pemphigus vulgaris antigen
• DSG3
• pemphigus vulgaris antigen
• PVA

Background

Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DSG3