Desmoglein-2 Antibody (JB96-37)

Western Blot: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Western blot analysis of Desmoglein 2 on Siha cell lysates using anti-Desmoglein 2 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Analysis of paraffin-embedded rat heart tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Analysis of A431 cells with Desmoglein 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Western Blot: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Analysis of Desmoglein 2 on A431 cell using anti-Desmoglein 2 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Analysis of paraffin-embedded human colon cancer tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Analysis of paraffin-embedded human prostate tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Analysis of paraffin-embedded human placenta tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody (JB96-37) [NBP2-75469] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Product Discontinued

Desmoglein-2 Antibody (JB96-37) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Desmoglein-2 aa 961-1,118 / 1,118. (SwissProt: Q14126 Human; SwissProt: O55111 Mouse; SwissProt: F1LYX9 Rat)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DSG2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Desmoglein-2 Antibody (JB96-37)

• ARVC10
• ARVD10
• Cadherin family member 5
• CDHF5
• CDHF5MGC117034
• CMD1BB
• desmoglein 2
• Desmoglein2
• Desmoglein-2
• DSG2
• HDGC
• MGC117036
• MGC117037

Background

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Desmoglein-2 Antibody (NBP2-75469). (Showing 1 - 1 of 1 FAQ).

1. I am wondering whether the product NBP2-75469 is good for live cell sorting. Thanks
   • It looks like the immunogen for this Desmoglein-2 antibody is in the C-terminus, which is located in the cytoplasm, so you probably would need to permeabilize the cells to use this antibody in Flow.

Bioinformatics

• Gene Symbol: DSG2