Desmocollin-3 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Desmocollin-3 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Desmocollin-3
  Arg136-Lys690
  Accession # Q14574
• Specificity: Detects human Desmocollin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Desmocollin-2 is observed and less than 1% cross-reactivity with rhDesmocollin-1 is ob
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-3 Antibody [Alexa Fluor® 750]

• Cadherin family member 3
• CDHF3
• CDHF3HT-CP
• desmocollin 3
• Desmocollin3
• Desmocollin-3
• Desmocollin-4
• DSC
• DSC1
• DSC2
• DSC3
• DSC4
• DSC4desmocollin-3
• HT-CP

Background

Desmocollin-3 is a 100‑110 kDa type I transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1‑3). The human Desmocollin-3 is synthesized with a 108 amino acid (aa) propeptide. The mature protein consists of a 555 aa extracellular domain (ECD) that contains five cadherin‑like domains, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (4, 5). Within the ECD, human Desmocollin-3 shares 78% aa sequence identity with mouse and rat Desmocollin-3. It shares 52% and 64% aa sequence identity with Desmocollin-1 and -2, respectively. A splice variant of human Desmocollin-3 has a truncated and substituted cytoplasmic domain (4, 6). Desmocollin-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (5, 7, 8). Desmocollin-1 and -2, by contrast, are preferentially localized in the more superficial layers (7). In mouse, Desmocollin-3 is expressed throughout embryogenesis and is required for non‑desmosomal functions during early development (9, 10). During apoptosis, both isoforms of Desmocollin-3 are proteolytically cleaved by caspases and metalloproteases (11). Desmocollin-3 can be up‑regulated or down‑regulated in carcinoma (6, 12, 13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.