Desmocollin-2 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Desmocollin-2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Desmocollin-2
  Arg136-Arg684
  Accession # Q02487
• Specificity: Detects human Desmocollin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Desmocollin-1 and rhDesmocollin-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DSC2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-2 Antibody

• ARVD11
• Cadherin family member 2
• CDHF2
• CDHF2DGII/III
• desmocollin 2
• Desmocollin2
• Desmocollin-2
• Desmocollin-3
• Desmosomal glycoprotein II
• desmosomal glycoprotein II/III
• Desmosomal glycoprotein III
• DG2
• DSC2
• DSC3DKFZp686I11137

Background

Desmocollin-2 (DSC2) is a 100-110 kDa transmembrane glycoprotein member of the cadherin family of calcium dependent adhesion molecules (1 - 3). Human DSC2 is synthesized as a 901 amino acid (aa) precursor. It contains a 108 aa propeptide plus a mature region that consists of a 559 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 186 aa cytoplasmic region (4). Within the ECD, human DSC2 shares 74% - 79% aa sequence identity with bovine, mouse, and rat DSC2. It shares 54% and 64% aa sequence identity with Desmocollin-1 and -3, respectively. DSC2 is also expressed as long and short splice forms which differ in their cytoplasmic regions (4, 5). The N-terminal two cadherin-like domains mediate homophilic interactions as well as heterophilic interactions with Desmoglein-2 (6). DSC2 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (1, 5, 7). In contrast to DSC1 and DSC3, DSC2 is also expressed in simple epithelia lining the gastrointestinal tract, liver, and kidney (5). During colon carcinogenesis however, DSC2 is downregulated, while DSC1 and DSC3 are upregulated (8). DSC2 is additionally expressed in the myocardium, and a variety of DSC2 mutations are associated with the cardiac arrhythmia disorder ARVC (5, 9).

1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
2. Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
4. Parker, A.E. et al. (1991) J. Biol. Chem. 266:10438.
5. Nuber, U.A. et al. (1995) Eur. J. Cell Biol. 66:69.
6. Syed, S. et al. (2002) Biochem. J. 362:317.
7. Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
8. Khan, K. et al. (2006) Br. J. Cancer 95:1367.
9. van Tintelen, J.P. et al. (2007) Curr. Opin. Cardiol. 22:185.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desmocollin-2 Antibody (AF4688)(2)

Publications using AF4688

• Jennifer J. Hill, Tammy-Lynn Tremblay, François Fauteux, Jie Li, Edwin Wang, Adriana Aguilar-Mahecha et al. Glycoproteomic Comparison of Clinical Triple-Negative and Luminal Breast Tumors Journal of Proteome Research 3/6/2015 [PMID: 25658377]
• Nie Z, Merritt A, Rouhi-Parkouhi M Membrane-impermeable cross-linking provides evidence for homophilic, isoform-specific binding of desmosomal cadherins in epithelial cells. J. Biol. Chem., 2010-11-22;286(3):2143-54. 2010-11-22 [PMID: 21098030] (ICC, Immunoprecipitation, Western Blot, Human)

Bioinformatics

• Gene Symbol: DSC2
• Uniprot:
  • Q02487(Human)