Recombinant Human Desert Hedgehog/Dhh Protein

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBC1-18407
    • Availability
      Product Discontinued

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Recombinant Human Desert Hedgehog/Dhh Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 23-198 of Human Desert Hedgehog/Dhh

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DHH
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM MES pH 5.5, 0.5 MM DTT, 20% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Desert Hedgehog/Dhh Protein

  • desert hedgehog (Drosophila) homolog
  • desert hedgehog homolog
  • desert hedgehog protein
  • Desert Hedgehog
  • DHH
  • HHG-3MGC35145

Background

Desert Hedgehog belongs to the hedgehog family. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N- terminal domain is the active species in both local and long-range signaling, whereas the C-terminal domain has no signaling activity. This protein is produced by Sertoli cells and may be involved in both male gonadal differentiation and perineurial development. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. Recombinant DHH protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DHH