Desert Hedgehog/Dhh Antibody [DyLight 350]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: DyLight 350

Desert Hedgehog/Dhh Antibody [DyLight 350] Summary

• Immunogen: E. coli-derived recombinant mouse Desert Hedgehog/Dhh
  aa 23-198
  Accession # Q61488
• Specificity: Detects mouse Desert Hedgehog/Dhh N-Terminus in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Shh N-terminal peptide (aa 25‑198) is observed. Additionally, in direct ELISAs, no cross‑reactivity with rmDhh C-terminal peptide (aa 199‑396) and rmIhh C-terminal peptide (aa 241‑449) is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DHH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Desert Hedgehog/Dhh Antibody [DyLight 350]

• desert hedgehog (Drosophila) homolog
• desert hedgehog homolog
• desert hedgehog protein
• Desert Hedgehog
• DHH
• HHG-3MGC35145

Background

Dhh encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHH