Desert Hedgehog/Dhh Antibody (RM0087-7C8)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
RM0087-7C8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Desert Hedgehog/Dhh Antibody (RM0087-7C8) Summary

Immunogen
Mouse recombinant dhh C-terminal fragment
Specificity
This was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse recombinant protein of dhh C-terminal fragment.
Isotype
IgG2
Clonality
Monoclonal
Host
Rat
Gene
DHH
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
This antibody detects mouse dhh C-terminal polypeptides, but not Nterminal one in Western blotting.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.2 ml sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Desert Hedgehog/Dhh Antibody (RM0087-7C8)

  • desert hedgehog (Drosophila) homolog
  • desert hedgehog homolog
  • desert hedgehog protein
  • Desert Hedgehog
  • DHH
  • HHG-3MGC35145

Background

This gene encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N terminal portion is soluble and contains the signalling activity while the C terminal portion is involved in precursor processing. More importantly, the C terminal product covalently attaches a cholesterol moiety to the N terminal product, restricting the N terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DHH
Entrez