DEC1 Antibody

Western Blot: DEC1 Antibody [NBP2-27151] - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with Dec1 antibody at 3 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-27151] - Staining of human brain tissue probed with Dec1 antibody at 5 ug/ml.

Product Discontinued

DEC1 Antibody Summary

• Immunogen: A portion of amino acids 20-88 of human Dec1 was used as the immunogen.
• Predicted Species: Mouse (96%), Rat (96%), Sheep (96%), Bovine (94%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BHLHE40
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 2-4ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for DEC1 Antibody

• basic helix-loop-helix domain containing, class B, 2
• basic helix-loop-helix family, member e40
• bHLHb2
• bHLHe40
• Class B basic helix-loop-helix protein 2
• class E basic helix-loop-helix protein 40
• DEC1HLHB2
• differentially expressed in chondrocytes 1
• Differentially expressed in chondrocytes protein 1
• differentiated embryo chondrocyte expressed gene 1
• Enhancer-of-split and hairy-related protein 2
• SHARP2
• SHARP-2
• Stimulated by retinoic acid gene 13 protein
• STRA13FLJ99214
• Stra14

Background

Dec1, which is also known as Stra13 or Sharp2, is a basic helix-loop-helix (bHLH) transcription factor that has been found to play an important role in circadian system. Dec1 contains one basic helix-loop-helix (bHLH) domain, one Orange domain and is reported to be expressed in Treg cells. It is also reported that Dec1 is required for long-term maintenance of Treg cells after adoptive transfer to suppress effector T cell-mediated inflammation. Dec1 positively regulates CD25 expression in association with Runx 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEC1 Antibody (NBP2-27151)(2)

Publications using NBP2-27151

• Hleihel R, Skayneh H, de The H et al. Primary cells from patients with adult T cell leukemia/lymphoma depend on HTLV-1 Tax expression for NF-kB activation and survival Research Square 2023-03-08 [PMID: 37137914] (Western Blot, Human)
• Donninger H, Calvisi DF, Barnoud T et al. NORE1A is a Ras senescence effector that controls the apoptotic/senescent balance of p53 via HIPK2 J. Cell Biol. 2015-03-16 [PMID: 25778922] (WB)
  
  Details:
  DEC1 antibody used for Western blot analysis of lysates from HBEC-3KT cells that were subjected or not to stable knocked down of NORE1A (Figure 1E).

Bioinformatics

• Gene Symbol: BHLHE40