DDX6 Antibody

Western Blot: DDX6 Antibody [NB100-2818] - Staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818] - Staining of paraffin embedded Human Thymus. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DDX6 Antibody Summary

• Description: Novus Biologicals Goat DDX6 Antibody (NB100-2818) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DDX6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-AEYHSEPVEDEKP corresponding to C-Terminus according to NP_004388.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DDX6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 50 kDa band observed in lysates of Daudi, Jurkat and HepG2 (calculated MW of 53.2 kDa band according to NP_004388.1).
• Control:

  Jurkat Whole Cell Lysate
  DDX6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX6 Antibody

• ATP-dependent RNA helicase p54
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• DEAD box protein 6
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
• EC 3.6.1
• EC 3.6.4.13
• FLJ36338
• HLR2DEAD box-6
• Oncogene RCK
• probable ATP-dependent RNA helicase DDX6
• RCKP54

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX6 Antibody (NB100-2818)(1)

Publication using NB100-2818

• Cougot N, Babajko S, Seraphin B. Cytoplasmic foci are sites of mRNA decay in human cells. J Cell Biol 2004-04-01 [PMID: 15067023]

Bioinformatics

• Gene Symbol: DDX6
• Entrez:
  • 1656(Human)
  • 13209(Mouse)
• Uniprot:
  • NP_004388.1(Human)