DDX19B Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

DDX19B Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human DDX19B.
  
  

  Source: E. coli

  Amino Acid Sequence: AAAESLSNLHLKEEKIKPDTNGAVVKTNANA

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: DDX19B
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-33462.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for DDX19B Recombinant Protein Antigen

• ATP-dependent RNA helicase DDX19
• DBP5ATP-dependent RNA helicase DDX19B
• DDX19DEAD-box protein 5
• DEAD (Asp-Glu-Ala-As) box polypeptide 19
• DEAD (Asp-Glu-Ala-As) box polypeptide 19B
• DEAD box protein 19B
• DEAD box RNA helicase DEAD5
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog)
• DEAD-box RNA helicase DEAD5
• EC 3.6.1
• EC 3.6.4.13
• RNAh
• yeast Dbp5 homolog

Background

DDX19B, also known as ATP-dependent RNA helicase DDX19B, consists of four isoforms of sizes 53.9 kDa, 50.5 kDa, 41.8 kDa, and 51 kDa and is involved in exporting mRNA from the nucleus by remodeling the ribonucleoprotein particles instead of unwinding RNA duplexes. Current research is linking the protein to diseases and disorders such as apraxia, pneumonia, Werner syndrome, malaria, and locked-in syndrome. The protein interacts in protein transport pathways with PRKAG1, CDC5L, RAE1, UBA5, and SNW1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DDX19B