Recombinant Human DCPS His Protein

SDS-Page: DCPS Protein [NBP1-72406] - 15% SDS-PAGE (3 ug protein loaded).

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human DCPS His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-337 of Human DCPS
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPDPSDGF VLIPDLKWNQ QQLDDLYLIA ICHRRGIRSL RDLTPEHLPL LRNILHQGQE AILQRYRMKG DHLRVYLHYL PSYYHLHVHF TALGFEAPGS GVERAHLLAE VIENLECDPR HYQQRTLTFA LRADDPLLKL LQEAQQS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DCPS
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 40.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human DCPS His Protein

• DCS1
• DCS-1
• decapping enzyme, scavenger
• EC 3.-
• heat shock-like protein 1
• HINT5
• HINT-5homolog of C. elegans 7meGMP-directed hydrolase dcs-1
• Hint-related 7meGMP-directed hydrolase
• Histidine triad protein member 5
• HSL1
• HSPC015
• mRNA decapping enzyme
• scavenger mRNA-decapping enzyme DcpS

Background

DCPS, also known as scavenger mRNA-decapping enzyme, is belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3'-5' degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain. Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DCPS