DCP2 Antibody (PCRP-DCP2-1D6)

Immunocytochemistry/Immunofluorescence: DCP2 Antibody (PCRP-DCP2-1D6) [NBP3-13864] - Immunofluorescent staining of PFA-fixed human cell line MCF7. DCP2 Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (green); phalloidin (red).

Flow Cytometry: DCP2 Antibody (PCRP-DCP2-1D6) [NBP3-13864] - Flow cytometric analysis of PFA-fixed HeLa cells. DCP2 antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Protein Array: DCP2 Antibody (PCRP-DCP2-1D6) [NBP3-13864] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DCP2 Antibody (PCRP-DCP2-1D6).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IP, MA
• Clone: PCRP-DCP2-1D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DCP2 Antibody (PCRP-DCP2-1D6) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14037)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human DCP2protein (Uniprot: Q8IU60)
• Localization: Cytoplasm, P. body, Nucleus. Predominantly cytoplasmic, in processing bodies (PB), A minor amount is nuclear
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCP2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Theoretical MW: 48.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for DCP2 Antibody (PCRP-DCP2-1D6)

• DCP2 decapping enzyme homolog (S. cerevisiae)
• EC 3.-
• FLJ33245
• hDpc
• mRNA-decapping enzyme 2
• nudix (nucleoside diphosphate linked moiety X)-type motif 20
• Nudix motif 20
• NUDT20Nucleoside diphosphate-linked moiety X motif 20

Background

DCP2 is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCP2
• Uniprot:
  • Q8IU60(Human)