DCIR/CLEC4A Antibody (9E8) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: DCIR/CLEC4A Antibody (9E8) - Chimeric [NBP3-09023] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of 9E8 (NBP3-09023) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09023, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: DCIR/CLEC4A Antibody (9E8) - Chimeric [NBP3-09023] - Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 9E8 (NBP3-09023, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 9E8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

DCIR/CLEC4A Antibody (9E8) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody. Clone 9E8 was used by HLDA to establish CD designation.
• Immunogen: This antibody was raised by immunising BALB/c mice with a dendritic cell immunoreceptor ectodomain-human Fc fusion protein.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CLEC4A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody has been used in flow cytometric analysis of DCIR expression by peripheral blood mononuclear cells (Klechevsky et al, 2010). A recombinant humanised form of this antibody fused to protein antigens is under investigation as an antigen-targeting vaccine (Flamar et al, 2012), and has been shown to trigger antigen-specific CD8+ T cell immunity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DCIR/CLEC4A Antibody (9E8) - Chimeric - Azide and BSA Free

• CD367
• CLEC4A
• CLECSF6
• CLECSF6C-type lectin superfamily member 6
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 6
• C-type lectin domain family 4 member A
• C-type lectin domain family 4, member A
• DCIR
• DCIRLLIR
• DDB27
• DDB27C-type lectin DDB27
• Dendritic cell immunoreceptor
• Lectin-like immunoreceptor
• LLIR

Background

CLEC4A, also known as DCIR, is a member of the C-type lectin/C-type lectin-like (CTL/CTLD) domain superfamily. Members of the CTL/CTLD family have a variety of functions including cell adhesion, glycoprotein turnover and cell-cell signaling. CLEC4A may play a role in regulating immune reactivity. This protein is currently being studied for research on the following diseases and disorders: rheumatoid arthritis, osteoarthritis, pneumonia, influenza, eclampsia and melanoma. CLEC4A is known to have interactions with PTPN6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC4A