DAZAP2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

DAZAP2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human DAZAP2.

  Source: E. coli

  Amino Acid Sequence: GASLYLPMAQSVAVGPLGSTIPMAYYPVGPIYPPGSTVLVEGGYDAGARFGAGATAGNIPPPPPGC

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: DAZAP2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-58696.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for DAZAP2 Recombinant Protein Antigen

• DAZ associated protein 2
• deleted in azoospermia associated protein 2
• Deleted in azoospermia-associated protein 2
• KIAA0058DAZ-associated protein 2
• MGC14319
• MGC766
• proline-rich transcript in brain
• proline-rich transcript, brain-expressed protein
• PRTB

Background

DAZAP2, also known as DAZ-associated protein2, consists of five isoforms of sizes 17.3 kDa, 16.1 kDa, 14 kDa, 9 kDa, and 22.8 kDa and is involved in interactions with DAZ proteins and transforming growth factor signaling molecules. This protein is being studied for connection to disorders and diseases such as multiple myeloma, brachydactyly, ataxia, prostatitis, and azoospermia. The protein interacts in pathways including cell signaling, transcription regulation, spermatogenesis, and RNA splicing, which also involve POLI, RHOXF2, BATF2, EXD3, and ZFAND2B proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DAZAP2