DARS2 Antibody - BSA Free

Images

 
Independent Antibodies: Western Blot: DARS2 Antibody [NBP1-83854] - Analysis using Anti-DARS2 antibody NBP1-83854 (A) shows similar pattern to independent antibody NBP1-83855 (B).
Immunocytochemistry/ Immunofluorescence: DARS2 Antibody [NBP1-83854] - Staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Staining of human tonsil shows moderate cytoplasmic-membranous positivity in non-germinal center cells and germinal center cells.
Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Independent Antibodies: Staining of human kidney, placenta, rectum and tonsil using Anti-DARS2 antibody NBP1-83854 (A) shows similar protein distribution across tissues to independent antibody NBP1-83855 (B).
Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
 

Independent Antibodies

       

Order Details

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Catalog# & Formulation Size Price

DARS2 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DARS2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DARS2 Protein (NBP1-83854PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DARS2 Antibody - BSA Free

  • aspartate tRNA ligase 2, mitochondrial
  • Aspartate--tRNA ligase
  • aspartyl-tRNA synthetase 2, mitochondrial
  • aspartyl-tRNA synthetase, mitochondrial
  • AspRS
  • ASPRS. LBSL
  • EC 6.1.1
  • EC 6.1.1.12
  • FLJ10514
  • LBSL
  • MT-ASPRS
  • RP3-383J4.2

Background

The protein encoded by the DARS2 gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DARS2