DAP Kinase 1 [p Ser308] Antibody (DKPS308)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP, MiAr
Clone
DKPS308
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.0 mg/ml

Order Details

DAP Kinase 1 [p Ser308] Antibody (DKPS308) Summary

Immunogen
Synthetic peptide, corresponding to amino acids 303-312 (pSER308) of Human DAP Kinase 1.
Modification
p Ser308
Localization
Cytoplasmic
Specificity
DAP Kinase 1 Phosphospecific (DKPS308) [Ser308]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DAPK1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Microarray
Application Notes
Dot: Use at a concentration of 1 - 2 ug/ml, Determined by immunoblotting using 293T (human embryonal kidney) cells transfected with DAPKinase expression vector.
Positive Control
DAP Kinase 1 Lysate (NBL1-09716)
Publications
Read Publication using
NB120-10524 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein A or G purified

Alternate Names for DAP Kinase 1 [p Ser308] Antibody (DKPS308)

  • DAPKDAP kinase 1
  • death-associated protein kinase 1
  • DKFZp781I035
  • EC 2.7.11
  • EC 2.7.11.1

Background

DAPK1 (Death-associated protein kinase 1) is a calmodulin dependent serine/threonine serine kinase which functions as a positive mediator of gamma-interferon induced programmed cell death. DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAP Kinase 1 Antibody (NB120-10524)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DAPK1
Entrez
Uniprot