DACT3/Dapper 3 Antibody

Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse DACT3/Dapper 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for DACT3/Dapper 3 at approximately 45 kDa with human brain and 50 kDa with mouse brain (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DACT3/Dapper 3 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse DACT3/Dapper 3
  Pro486-Lys587
  Accession # Q0PHV7
• Specificity: Detects human and mouse DACT3/Dapper 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DACT2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DACT3
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DACT3/Dapper 3 Antibody

• Antagonist of beta-catenin Dapper homolog 3
• arginine rich region 1
• Arginine-rich region 1 protein
• DACT3
• Dapper 3
• dapper homolog 3
• dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis)
• MGC15476
• RRR1

Background

Dapper 3 (also Dact 3) is a 63 kDa (predicted) member of the DACT (Dapper/Frodo) family of Dvl-binding proteins.  It is expressed in cerebral cortex and uterus where it antagonizes Wnt/ beta -catenin signaling.  When present in colorectal tumor cells, it initiates apoptosis.  Both effects are likely the result of a Dapper 3 interaction with Dvl2, thereby blocking beta -catenin entry into the nucleus.  Mouse Dapper 3 is 610 amino acids (aa) in length It contains one coiled-coil region (aa 63-87), an Arg-rich domain (aa 351-476), and a PZD-binding motif (aa 607-610).  There is one potential splice variant that shows a 45 aa substitution for aa 413-610.  In human Dact 3 the frameshift at position 119 is reported to yield a protein product with estimated moleqular weight of 41 kDa. Over aa 486-587, mouse dapper 3 shares 82% and 99% aa identity with human and rat dapper 3, respectively.      

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Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DACT3
• Uniprot:
  • Q0PHV7()