DACT3/Dapper 3 Antibody (2A5) - BSA Free

Western Blot: DACT3/Dapper 3 Antibody (2A5) [NBP1-19052] - Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry-Paraffin: DACT3/Dapper 3 Antibody (2A5) [NBP1-19052] - Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P
• Clone: 2A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DACT3/Dapper 3 Antibody (2A5) - BSA Free Summary

• Immunogen: Recombinant human DACT3/Dapper 3 (89-202aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DACT3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:250-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for DACT3/Dapper 3 Antibody (2A5) - BSA Free

• Antagonist of beta-catenin Dapper homolog 3
• arginine rich region 1
• Arginine-rich region 1 protein
• DACT3
• Dapper 3
• dapper homolog 3
• dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis)
• MGC15476
• RRR1

Background

DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications. Anti-human DACT3 mAb, clone 2A5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DACT3 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DACT3
• Entrez:
  • 147906(Human)
• Uniprot:
  • NP_659493(Human)