DACT3/Dapper 3 Antibody (2A5) - BSA Free

Images

 
Western Blot: DACT3/Dapper 3 Antibody (2A5) [NBP1-19052] - Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were ...read more
Immunohistochemistry-Paraffin: DACT3/Dapper 3 Antibody (2A5) [NBP1-19052] - Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

DACT3/Dapper 3 Antibody (2A5) - BSA Free Summary

Immunogen
Recombinant human DACT3/Dapper 3 (89-202aa) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DACT3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:250-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for DACT3/Dapper 3 Antibody (2A5) - BSA Free

  • Antagonist of beta-catenin Dapper homolog 3
  • arginine rich region 1
  • Arginine-rich region 1 protein
  • DACT3
  • Dapper 3
  • dapper homolog 3
  • dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis)
  • MGC15476
  • RRR1

Background

DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications. Anti-human DACT3 mAb, clone 2A5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DACT3 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24768
Species: Ch, Hu, Mu, Pm, Rt, Xp, Ze
Applications: IHC, IHC-P, WB
NBP2-24648
Species: Hu, Mu, Pm, Rt
Applications: WB
NBP1-76955
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-33596
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PAGE, WB
NB100-56340
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NB100-56745
Species: Hu, Mu
Applications: ChIP, ChIP, ICC/IF, IHC, IHC-P, IP, WB
NBP1-87511
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-45646
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-54467
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
H00006932-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
H00011262-M07
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA
H00004999-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
MAB2337
Species: Hu
Applications: IP, WB
NBP2-03367
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB300-560
Species: Av, Bv, Ma, Hu, Mu, Pm, Rt
Applications: IF, IHC, IHC-Fr, IHC-P, WB
NBP2-34050
Species: Hu
Applications: IHC, IHC-P
NBP2-49145
Species: Hu
Applications: IHC, IHC-P
NBP1-19052
Species: Hu
Applications: WB, ELISA, IHC

Publications for DACT3/Dapper 3 Antibody (NBP1-19052) (0)

There are no publications for DACT3/Dapper 3 Antibody (NBP1-19052).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DACT3/Dapper 3 Antibody (NBP1-19052) (0)

There are no reviews for DACT3/Dapper 3 Antibody (NBP1-19052). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DACT3/Dapper 3 Antibody (NBP1-19052) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DACT3/Dapper 3 Products

Diseases for DACT3/Dapper 3 Antibody (NBP1-19052)

Discover more about diseases related to DACT3/Dapper 3 Antibody (NBP1-19052).
 

Pathways for DACT3/Dapper 3 Antibody (NBP1-19052)

View related products by pathway.

PTMs for DACT3/Dapper 3 Antibody (NBP1-19052)

Learn more about PTMs related to DACT3/Dapper 3 Antibody (NBP1-19052).
 

Research Areas for DACT3/Dapper 3 Antibody (NBP1-19052)

Find related products by research area.

Blogs on DACT3/Dapper 3

There are no specific blogs for DACT3/Dapper 3, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DACT3/Dapper 3 Antibody (2A5) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol DACT3
Entrez
Uniprot