DACT3/Dapper 3 Antibody (2A5)

Images

 
Western Blot: DACT3/Dapper 3 Antibody (2A5) [NBP1-19052] - Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were ...read more
Immunohistochemistry-Paraffin: DACT3/Dapper 3 Antibody (2A5) [NBP1-19052] - Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

DACT3/Dapper 3 Antibody (2A5) Summary

Immunogen
Recombinant human DACT3 (89-202aa) purified from E. coli
Specificity
DACT3 (2A5)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DACT3
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for DACT3/Dapper 3 Antibody (2A5)

  • Antagonist of beta-catenin Dapper homolog 3
  • arginine rich region 1
  • Arginine-rich region 1 protein
  • DACT3
  • Dapper 3
  • dapper homolog 3
  • dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis)
  • MGC15476
  • RRR1

Background

DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications. Anti-human DACT3 mAb, clone 2A5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DACT3 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol DACT3
Entrez
Uniprot