D4S234E Antibody

Images

 
Western Blot: D4S234E Antibody [NBP2-26148] - NBP2-26148 (0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: D4S234E Antibody [NBP2-26148] - (3ug/ml) staining of primary DIV9 cells from Hippocampus E18 Rat embryos showed exclusive localization (red, Alexa 568) within the neurons (MAP2 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

D4S234E Antibody Summary

Immunogen
Peptide with sequence EKGTKQPLLEDGFD-C corresponding to N-Terminus according to NP_077042.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NSG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
Western blot: Approx 22kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9kDa according to NP_035072.2). Primary incubation was 1 hour.
Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only.
Publications
Read Publication using NBP2-26148.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for D4S234E Antibody

  • carboxyterminally EE-tagged neuron-enriched endosomal 21 kDa protein
  • D4S234Brain neuron cytoplasmic protein 1
  • DNA segment on chromosome 4 (unique) 234 expressed sequence
  • NEEP21
  • neuron-specific protein family member 1
  • NSG1
  • P21

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for D4S234E Antibody (NBP2-26148)(1)

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Bioinformatics

Gene Symbol NSG1
Entrez
Uniprot
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