Cytokeratin, LMW Antibody (KRTL/1077) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - IHC-Prediluted [NBP2-48269] - Formalin-fixed, paraffin-embedded Rat Stomach with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - IHC-Prediluted [NBP2-48269] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, LMW Monoclonal Antibody (KRTL/1077).

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - IHC-Prediluted [NBP2-48269] - Formalin-fixed, paraffin-embedded Rat Oviduct stained with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).

• Reactivity: Hu, Rt
• Applications: IHC
• Clone: KRTL/1077
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Cytokeratin, LMW Antibody (KRTL/1077) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant human Cytokeratin, LMW protein (Uniprot: Q7Z794)
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT77
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Shows broad species reactivity.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin, LMW Antibody (KRTL/1077) - IHC-Prediluted

• Cytokeratin LMW
• cytokeratin-1B
• keratin 77
• keratin, type II cytoskeletal 1b
• KRT77
• type-II keratin Kb39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT77