Cytokeratin 8 Antibody (C-43)

Product Discontinued

Cytokeratin 8 Antibody (C-43) Summary

• Immunogen: Cytoskeleton preparation from HeLa human cervix carcinoma cell line. (NM_002273.3).
• Marker: Epithelial Cell Marker
• Specificity: The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Predicted molecular weight 52. 5 kDa.
• Control:

  Cytokeratin 8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 8 Antibody (C-43)

• CARD2
• CK8
• CK-8
• CYK8
• Cytokeratin 8
• Cytokeratin-8
• EndoA
• K2C8
• K8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• KRT8
• Type-II keratin Kb8

Background

Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 8 Antibody (NB500-350)(3)

Publications using NB500-350

• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (Human)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Leow CC, Wang B-e, Ross J et al. Prostate-specific Klf6 Inactivation Impairs Anterior Prostate Branching Morphogenesis through Increased Activation of the Shh Pathway. J Biol Chem;284(31):21057-21065. 2009-01-01 [PMID: 19494112]

Bioinformatics

• Gene Symbol: KRT8
• Entrez:
  • 3856(Human)
  • 16691(Mouse)
  • 25626(Rat)
• Uniprot:
  • P05787(Human)