Human Cytokeratin 6a ELISA Kit (Colorimetric)

• Reactivity: Hu
• Suitable Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Standard Curve Range: 15.6-1000 pg/mL
• Sensitivity: 6.1 pg/mL

Human Cytokeratin 6a ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 15.6-1000 pg/mL
• Sensitivity: 6.1 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12
• Intra-Assay: %CV < 10
• Spike Recovery: 85 - 106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KRT6A

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cytokeratin 6a ELISA Kit (Colorimetric)

• Allergen Hom s 5
• CK6A
• CK-6A
• CK6C
• CK6D
• CK-6D
• cytokeratin 6A
• cytokeratin 6C
• cytokeratin 6D
• Cytokeratin-6A
• Cytokeratin-6D
• K6A
• K6C
• K6D
• keratin 6A
• keratin 6C
• keratin 6D
• keratin, epidermal type II, K6A
• keratin, type II cytoskeletal 6A
• Keratin-6A
• KRT6C
• KRT6D
• Type-II keratin Kb6

Background

The human type II Cytokeratin 6 (CK6; 56 kDa) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly the dominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and that the various isoforms are differentially regulated within and between epithelial tissue types (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6, CK6a and MK6b. These genes display a complex expression pattern with constitutive expression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyonychia congenital (PC) is characterized by hypertrophic nail dystrophy and associated ectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16, whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: KRT6A