Cytokeratin 3 Antibody (KRT3/2542) [Janelia Fluor® 646]

• Reactivity: Hu, Bv, Rb
• Applications: WB, IHC
• Clone: KRT3/2542
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

Cytokeratin 3 Antibody (KRT3/2542) [Janelia Fluor® 646] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.
• Localization: Cytoplasmic
• Marker: Corneal Epithelial Marker
• Specificity: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins, which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmanns Corneal Dystrophy.KRT3/2542 recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-08582. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Cytokeratin 3 Antibody (KRT3/2542) [Janelia Fluor® 646]

• CK3
• CK-3
• cytokeratin-3
• FLJ95909
• K3cytokeratin 3
• keratin 3
• keratin, type II cytoskeletal 3,65 kDa cytokeratin
• Keratin-3
• Type-II keratin Kb3

Background

The protein encoded by the Cytokeratin 3 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT3