Cytochrome P450 4A Antibody

Product Discontinued

Cytochrome P450 4A Antibody Summary

• Immunogen: Synthetic peptide: DPSRFAPDSPRHSHS, corresponding to amino acids 431-445 of Rat Cytochrome P450 4A.
• Specificity: Detects Cytochrome P450 4A (isoforms 1, 2, & 3)from feline, rabbit and rat tissues. This detects, to a lesser extent, isoforms 4A1 and 4A3.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP4A11
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1500
• Application Notes: Western blot: use at a dilution of 1/1500. Immunohistochemistry: use at a dilution of 1/100.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~50 kDa protein representing P450 4A in rat liver extract. In the same sample, a slightly larger band representing 4A3 is also clearly visible. Immunohistochemical staining of P450 4A in rat brain results in perivascular and surrounding cortex staining. Immunoprecipitation was reported in scientific literature.

Reactivity Notes

Cross-reacts with Rabbit, Rat and Cat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Cytochrome P450 4A Antibody

• 20-HETE synthase
• CP4Y
• CYP4A2
• cytochrome P450 4A11
• cytochrome P450, family 4, subfamily A, polypeptide 11
• cytochrome P450, subfamily IVA, polypeptide 11
• cytochrome P-450HK-omega
• cytochrome P450HL-omega
• fatty acid omega-hydroxylase
• lauric acid omega-hydroxylase
• P450HL-omega

Background

The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA's which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 4A Antibody (NB300-609)(6)

Publications using NB300-609

• Hagemeyer CE et al. 7-Benzyloxyresorufin-O-dealkylase activity as a marker for measuring cytochrome P450 CYP3A induction in mouse liver. Anal Biochem;398(1):104-11. 2010-03-01 [PMID: 19903449] (WB)
• Anthonio EA et al. Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6. BMC Cell Biol;10():58. 2009-08-17 [PMID: 19686593] (WB)
• Marvar PJ et al. High dietary salt reduces the contribution of 20-HETE to arteriolar oxygen responsiveness in skeletal muscle. Am J Physiol Heart Circ Physiol;292(3):H1507-15. 2007-03-01 [PMID: 17114243] (WB)
• Fransen M et al. Potential role for Pex19p in assembly of PTS-receptor docking complexes. J Biol Chem;279(13):12615-24. 2004-03-26 [PMID: 14715663] (IP)
• Graham RA et al. In vivo and in vitro induction of cytochrome P450 enzymes in beagle dogs. Drug Metab Dispos;30(11):1206-13. 2002-11-01 [PMID: 12386126] (WB)
• Ma YH et al. Altered renal P-450 metabolism of arachidonic acid in Dahl salt-sensitive rats. Am J Physiol;267(2 Pt 2):R579-89. 1994-08-01 [PMID: 8067471] (WB)

Bioinformatics

• Gene Symbol: CYP4A11
• Entrez:
  • 24306(Rat)
• Uniprot:
  • P20816(Rat)