Cytochrome P450 2E1 Antibody (JM10-85)

Western Blot: Cytochrome P450 2E1 Antibody (JM10-85) [NBP2-67021] - Analysis of CYP2E1 on human liver cells lysates using anti-CYP2E1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (JM10-85) [NBP2-67021] - The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (JM10-85) [NBP2-67021] - Analysis of paraffin-embedded human liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (JM10-85) [NBP2-67021] - The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (JM10-85) [NBP2-67021] - Analysis of paraffin-embedded human liver cancer tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (JM10-85) [NBP2-67021] - Analysis of paraffin-embedded human liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (JM10-85) [NBP2-67021] - Analysis of paraffin-embedded mouse liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.

Product Discontinued

Cytochrome P450 2E1 Antibody (JM10-85) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Cytochrome P450 2E1 aa 71-114 / 439. (SwissProt: P05181 Human; SwissProt: Q05421 Mouse; SwissProt: P05182 Rat)
• Localization: Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CYP2E1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytochrome P450 2E1 Antibody (JM10-85)

• 4-nitrophenol 2-hydroxylase
• CPE1
• CYP2Ecytochrome P450 2E1
• CYPIIE1
• cytochrome P450, family 2, subfamily E, polypeptide 1
• cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
• Cytochrome P450-J
• EC 1.14.13.-
• EC 1.14.13.n7
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• microsomal monooxygenase
• P450C2E
• P450-J
• xenobiotic monooxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP2E1