Cytochrome P450 1A1 Antibody

Product Discontinued

Cytochrome P450 1A1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 71-120 of Human CYP1A1
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of CYP1A1/2 (R98) pAb in extracts from RAW264.7 cells.
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 1A1 Antibody

• AHH
• AHRR
• aryl hydrocarbon hydroxylase
• CP11
• CYP1
• CYP1A1
• CYPIA1
• cytochrome P1-450, dioxin-inducible
• Cytochrome P450 1A1
• Cytochrome P450 form 6
• cytochrome P450, family 1, subfamily A, polypeptide 1
• Cytochrome P450-C
• Cytochrome P450-P1
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• P1-450
• P450-C
• P450DX
• subfamily I (aromatic compound-inducible), polypeptide 1
• xenobiotic monooxygenase

Background

This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 1A1 Antibody (NBP1-19551)(3)

Publications using NBP1-19551

• Hong CH, Lee CH, Yu HS, Huang SK. Benzopyrene, a major polyaromatic hydrocarbon in smoke fume, mobilizes Langerhans cells and polarizes Th2/17 responses in epicutaneous protein sensitization through the aryl hydrocarbon receptor. Int. Immunopharmacol. 2016-04-26 [PMID: 27129092] (ICC/IF, Human, Mouse)
• Garcia PV, Apolinario LM, Bockelmann PK et al. Alterations in ubiquitin ligase Siah-2 and its corepressor N-CoR after P-MAPA immunotherapy and anti-androgen therapy: new therapeutic opportunities for non-muscle invasive bladder cancer Int J Clin Exp Pathol. 2015-07-20 [PMID: 26191134] (WB, Rat)
• Bartikova H, Bousova I, Jedlickova P et al. Effect of standardized cranberry extract on the activity and expression of selected biotransformation enzymes in rat liver and intestine. Molecules. 2014-09-20 [PMID: 25237750]

Bioinformatics

• Gene Symbol: CYP1A1
• Entrez:
  • 1543(Human)
  • 13076(Mouse)
  • 25147(Rat)
• Uniprot:
  • P04798(Human)