Cystatin SN Antibody (213139)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 213139
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cystatin SN Antibody (213139) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cystatin SN
  Trp21-Ser141 (predicted)
  Accession # P01037
• Specificity: Detects human Cystatin SN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cystatins A, B, C, D, S, SA, E/M, rhFetuin A, rhFetuin B, rhHPRG, or rhThrombin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CST1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin SN Antibody (213139)

• CST1
• cystain-SA-I
• cystatin 1
• cystatin SA-I
• Cystatin SN
• cystatin-1
• cystatin-SN
• Salivary cystatin-SA-1
• type 2 family

Background

Cystatin SN is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SA, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SN inhibits members of the papain family including cathepsins B, C, H and L (3).

1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
2. Baron, A.C. et al. (1999) Oral Dis. 5:344.
3. Baron, A. et al. (1999) Oral Dis. 5:234.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin SN Antibody (MAB1285)(2)

Publications using MAB1285

• Yanzhen Lai, Yu Wang, Yaxian Wu, Meng Wu, Shan Xing, Ying Xie et al. Identification and Validation of Serum CST1 as a Diagnostic Marker for Differentiating Early-Stage Non-Small Cell Lung Cancer from Pulmonary Benign Nodules Cancer Control 6/2/2022 [PMID: 35653624]
• Ito T, Komiya-Ito A, Okuda K, Minaguchi K, Saitoh E, Yamada S, Kato T Murine monoclonal antibody which can distinguish cystatins SA1 and SA2. Mol. Immunol., 2005-01-08;42(10):1259-63. 2005-01-08 [PMID: 15829315] (ELISA Development, Western Blot, Human)

FAQs for Cystatin SN Antibody (MAB1285). (Showing 1 - 1 of 1 FAQs).

1. I notice you sell both antibodies and recombinant protein for human cystatin SN. Could you perhaps develop an ELISA for this protein?
   • NBP1-55995 and H00001469-B01P are both suitable for use as antibodies in ELISA. While a kit is not in development, you could use these to make your own ELISA kit, as many of our users do.

Bioinformatics

• Gene Symbol: CST1
• Uniprot:
  • P01037()