Cystatin F Antibody (292103) [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Clone: 292103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

Cystatin F Antibody (292103) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human Cystatin F in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E/M, S, recombinant mouse (rm) Cystatin/Stefin Homolog, rhFetuin A, rhFetuin B, rhHPRG, rhKininogen, or rhKininostatin is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin F Antibody (292103) [Alexa Fluor™ Plus 680]

• CMAP
• CST7
• Cystatin 7
• cystatin F (leukocystatin)
• Cystatin F
• Cystatin-7
• cystatin-F
• Cystatin-like metastasis-associated protein
• Leukocystatin

Background

Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a member of the cystatin superfamily (1‑3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards cathepsin L and legumain (1, 2, 5). Compared to other secreted cystatins including C, D, E/M, S, SA and SN, which contain two intra disulfide bonds, cystatin F has two extra Cys residues that may be involved in inter disulfide bonds. Indeed, rhCystatin F showed disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human cystatin F and glutathione S-transferase (2).

1. Ni, J. et al. (1998) J. Biol. Chem. 273:24797.
2. Halfon, S. et al. (1998) J. Biol. Chem. 273:16400.
3. Utsunomiya, T. et al. (2002) Clin Cancer 8:2591
4. Werle, B. et al. (2003) Biol. Chem. 384:281.
5. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.