Cystatin B/Stefin B Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Cystatin B/Stefin B Antibody [NBP1-31838] - Paraformaldehyde-fixed A431, using Cystatin B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at ...read more
Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838] - Paraffin-embedded tonsil. Cystatin B antibody dilution: 1:100.
Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-31838] - Paraffin-embedded mouse brain. Cystatin B antibody diluted at 1:100.

Product Details

Summary
Reactivity Hu, Mu, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
6.04 mg/ml

Order Details

Cystatin B/Stefin B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cystatin B. The exact sequence is proprietary.
Localization
Nucleus/Cytoplasm
Predicted Species
Primate (98%), Rhesus Macaque (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSTB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%), Pig (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
6.04 mg/ml
Purity
Protein A purified

Alternate Names for Cystatin B/Stefin B Antibody

  • CPI-B
  • CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B
  • CSTB
  • cystatin B (stefin B)
  • Cystatin B
  • EPM1
  • Liver thiol proteinase inhibitor
  • PME
  • Stefin B
  • stefin-B

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CSTB
Entrez