Cystatin B/Stefin B Antibody (227818)

• Reactivity: Mu
• Applications: WB, IP
• Clone: 227818
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Cystatin B/Stefin B Antibody (227818) Summary

• Immunogen: E. coli-derived recombinant mouse Cystatin B
  Met2-Phe98
  Accession # Q62426
• Specificity: Detects mouse Cystatin B in direct ELISAs and Western blots. In Western blots, 20‑25% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin D, S, SA, SN, recombinant mouse (rm) Cystatin A, E/M, rmFetuin A, rhFetuin B, rhHPRG, or rhKininogen is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CSTB
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin B/Stefin B Antibody (227818)

• CPI-B
• CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B
• CSTB
• cystatin B (stefin B)
• Cystatin B
• EPM1
• Liver thiol proteinase inhibitor
• PME
• Stefin B
• stefin-B

Background

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).

1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
2. Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
3. Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.
4. Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin B/Stefin B Antibody (MAB1409)(1)

Publication using MAB1409

• Cathepsin-B activity initiates apoptosis via digestive protease activation in pancreatic acinar cells and experimental pancreatitis J Biol Chem, 2016-05-12;0(0):. 2016-05-12 [PMID: 27226576] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CSTB
• Uniprot:
  • Q62426()