Cystatin B/Stefin B Antibody (227818) [Alexa Fluor™ Plus 680]

• Reactivity: Mu
• Clone: 227818
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 680

Cystatin B/Stefin B Antibody (227818) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects mouse Cystatin B in direct ELISAs and Western blots. In Western blots, 20‑25% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin D, S, SA, SN, recombinant mouse (rm) Cystatin A, E/M, rmFetuin A, rhFetuin B, rhHPRG, or rhKininogen is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin B/Stefin B Antibody (227818) [Alexa Fluor™ Plus 680]

• CPI-B
• CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B
• CSTB
• cystatin B (stefin B)
• Cystatin B
• EPM1
• Liver thiol proteinase inhibitor
• PME
• Stefin B
• stefin-B

Background

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).

1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
2. Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
3. Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.
4. Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.