Cyr61/CCN1 Antibody (466305) [Unconjugated]

Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 µg/mL Rat Anti-Mouse Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB4864) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 466305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cyr61/CCN1 Antibody (466305) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Cyr61/CCN1
  Asp176-Gly281
  Accession # P18406
• Specificity: Detects mouse Cyr61/CCN1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Cyr61 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CYR61
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyr61/CCN1 Antibody (466305) [Unconjugated]

• CCN family member 1
• CCN1
• CCN1IGF-binding protein 10
• Cyr61
• Cysteine-rich angiogenic inducer 61
• cysteine-rich, angiogenic inducer, 61
• GIG1
• GIG1cysteine-rich heparin-binding protein 61
• IBP-10
• IGFBP-10
• IGFBP10cysteine-rich, anigogenic inducer, 61
• Insulin-like growth factor-binding protein 10
• protein CYR61
• Protein GIG1

Background

Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins  alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176-281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyr61/CCN1 Antibody (MAB4864)(2)

Publications using MAB4864

• Luqiang Wang, Lutian Yao, Hao Duan, Fan Yang, Maohuan Lin, Rongxin Zhang et al. Plasminogen Regulates Fracture Repair by Promoting the Functions of Periosteal Mesenchymal Progenitors Journal of Bone and Mineral Research 11/1/2021 [PMID: 34378815]
• H Duan, Z He, M Lin, Y Wang, F Yang, RA Mitteer, HJ Kim, E Yeo, H Han, L Qin, Y Fan, Y Gong Plasminogen regulates mesenchymal stem cell-mediated tissue repair after ischemia through Cyr61 activation JCI Insight, 2020-08-06;5(15):. 2020-08-06 [PMID: 32759492] (Functional Assay, Neutralization, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CYR61
• Uniprot:
  • P18406()