Recombinant Human Cyclophilin-F His Protein

SDS-Page: Recombinant Human Cyclophilin-F Protein [NBC1-18533] - Cyclophilin F(PPIF), 21 kDa (198aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: WB, IP, PAGE, Bioactivity
• Concentration: 1 mg/ml

Recombinant Human Cyclophilin-F His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-207 of Human Cyclophilin-F
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS

• Details of Functionality: Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPIF
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Immunoprecipitation
  • SDS-Page
  • Western Blot
• Application Notes: Use in immunoprecipitation, and western blot reported in scientific literature (PMID 26387735)
• Theoretical MW: 21.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Analogous biological activity in mouse tissues/cells reported in scientific literature (PMID: 26387735)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Cyclophilin-F His Protein

• Cyclophilin F
• CYP3FLJ90798
• Cyp-D
• hCyP3
• MGC117207
• mitochondrial
• peptidyl-prolyl cis-trans isomerase, mitochondrial
• peptidylprolyl isomerase F (cyclophilin F)
• peptidylprolyl isomerase F

Background

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Cyclophilin-F Recombinant Protein (NBC1-18533)(1)

Publication using NBC1-18533

• Shanmughapriya S, Rajan S, Hoffman NE et al. SPG7 Is an Essential and Conserved Component of the Mitochondrial Permeability Transition Pore. Cell Press 2015-10-01 [PMID: 26387735] (WB, IP, Human, Mouse)

Bioinformatics

• Gene Symbol: PPIF