Human Cyclophilin 40 ELISA Kit (Colorimetric)

ELISA: Human Cyclophilin 40 ELISA Kit (Colorimetric) [NBP2-60549] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 12.5 - 200 ng/mL (example only; lot dependent)
• Sensitivity: 6.5 ng/mL (example only; lot dependent)

Human Cyclophilin 40 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 12.5 - 200 ng/mL (example only; lot dependent)
• Sensitivity: 6.5 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10.0% (example only; lot dependent)
• Intra-Assay: %CV < 5.4% (example only; lot dependent)
• Spike Recovery: 87 - 112% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PPID

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cyclophilin 40 ELISA Kit (Colorimetric)

• 40 kDa peptidyl-prolyl cis-trans isomerase D
• 40 kDa peptidyl-prolyl cis-trans isomerase
• cyclophilin D
• cyclophilin-40
• Cyclophilin-related protein
• CYP40
• CYP-40cyclophilin 40
• CYPD
• EC 5.2.1.8
• MGC33096
• peptidyl-prolyl cis-trans isomerase D
• peptidylprolyl isomerase D (cyclophilin D)
• peptidylprolyl isomerase D
• PPIase D
• Rotamase D

Background

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Cyclophilin 40 ELISA Kit (NBP2-60549). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: PPID