Cyclophilin 40 Antibody

Western Blot: Cyclophilin 40 Antibody [NB120-3567] - Analysis of rat heart extract.

Western Blot: Cyclophilin 40 Antibody [NB120-3567] - Analysis of rat brain extract.

Product Discontinued

Cyclophilin 40 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues S(42) G N P L V Y L D V D A N(54) C of mature human CyPD.
• Localization: Mitochondrial
• Specificity: NB120-3567 detects cyclophilin D (CyPD) in rat, canine, mouse, and human tissues.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPID
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
• Application Notes: Successful WB usage. WB: Detects an approx. 15 kDa protein in rat liver and heart extract, and a ~37 kDa in rat brain extract.
• Control:

  Cyclophilin 40 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cyclophilin 40 Antibody

• 40 kDa peptidyl-prolyl cis-trans isomerase D
• 40 kDa peptidyl-prolyl cis-trans isomerase
• cyclophilin D
• cyclophilin-40
• Cyclophilin-related protein
• CYP40
• CYP-40cyclophilin 40
• CYPD
• EC 5.2.1.8
• MGC33096
• peptidyl-prolyl cis-trans isomerase D
• peptidylprolyl isomerase D (cyclophilin D)
• peptidylprolyl isomerase D
• PPIase D
• Rotamase D

Background

Cyclophilin D (CyPD) is a member of the immunophilin family of proteins. Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyPD is located in the inter-membrane space of the mitochondria where it has been shown to be an integral member of the permeability transition pore complex which also includes the voltage dependent anion channel (VDAC) and the adenine nucleotide translocator (ANT).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPID
• Entrez:
  • 5481(Human)
  • 67738(Mouse)
  • 361967(Rat)